StockOptions MES 02 pH 5.3, 185 ml

– 185 ml volume
– Sterile filtered
– Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

HR2-943-**
** Refers to the reagent number in the StockOptions MES Buffer kit.
For example, reagent number 1 = HR2-943-01.

MES monohydrate
Synonyms: MES, 2-(N-morpholino)ethanesulfonic acid
or 4-morpholineethanesulfonic acid monohydrate
C6H13NO4S · H2O
Mr 213.25
CAS Number [145224-94-8]
EC Number 224-632-3
Beilstein Registry Number 6350956
RTECS QE3479500
MDL Number MFCD00149409
PubChem Substance ID 24885996
Purity >99.0%
pKa 6.1 at 25°C

Solutions are stable at 2-8°C for months. Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. When MES solutions are stored at room temperature, they can turn yellow within six months.

Useful pH range of MES 5.2 – 7.1

MES buffers formulated in Type 1+ ultrapure water, 18.2 megohm resistivity at 25°C, < 5 ppb Total Organic Carbon, <1 Bacteria (CFU/ml), <0.03 Endotoxin (EU/ml). The pH is adjusted using Sodium hydroxide (NaOH). Buffers are sterile filtered using 0.22 micron filters under sterile conditions

Ca < 0.005%
Cd < 0.0005%
Cl < 0.005%
Co < 0.0005%
Cr < 0.0005%
Cu < 0.0005%
Fe < 0.0005%
K < 0.005%
Mg < 0.0005%
Mn < 0.0005%
Na < 0.005%
Ni < 0.0005%
Pb < 0.0005%
SO4 < 0.005%
Zn < 0.0005%


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