Proti-Ace Reagent 01 (pack of 3 tubes), 1/ea

– Lyophilized enzymes for enhanced stability

Individual reagents from the Proti-Ace and Proti-Ace 2 kits. Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.

The unique freeze dried formulation of the Proti-Ace kit offers a much improved protease stability compared to liquid protease formulations. Each tube contains 0.1 mg of lyophilized enzyme and digest buffer as shown below. Add 100 microliters of deionized water (Type 1+) to each tube of lyophilized content to create a 1 mg/ml protease solution in digest buffer. Proti-Ace Dilution buffer is supplied as a ready to use solution.

Proti-Ace Reagent 1* (Qty 3):
• 1 mg/ml a-Chymotrypsin, 1 mM Hydrochloric acid, 2 mM Calcium chloride dihydrate
Proti-Ace Reagent 2* (Qty 3):
• 1 mg/ml Trypsin, 1 mM Hydrochloric acid, 2 mM Calcium chloride dihydrate
Proti-Ace Reagent 3* (Qty 3):
• 1 mg/ml Elastase, 200 mM Tris pH 8.0
Proti-Ace Reagent 4* (Qty 3):
• 1 mg/ml Papain, 2 mM EDTA, 2 mM TCEP hydrochloride, 2 mM L-Cysteine
Proti-Ace Reagent 5* (Qty 3):
• 1 mg/ml Subtilisin, 10 mM Sodium acetate trihydrate pH 7.5, 5 mM Calcium acetate hydrate
Proti-Ace Reagent 6* (Qty 3):
• 1 mg/ml Endoproteinase Glu-C (in deionized water)
* When 100 µl of deionized water is added to each Proti-Ace freeze dried reagent

Proti-Ace Dilution Buffer (Qty 3):
• 1.6 ml of 10 mM HEPES pH 7.5, 500 mM Sodium chloride

Proti-Ace 2 Reagent 1* (Qty 3):
• 1 mg/ml Proteinase K, 10 mM TRIS hydrochloride pH 7.5, 1 mM Calcium acetate hydrate
Proti-Ace 2 Reagent 2* (Qty 3):
• 1 mg/ml Clostripain (Endoproteinase-Arg-C), 10 mM HEPES pH 7.5, 2 mM TCEP hydrochloride, 2 mM L-Cysteine, 1 mM Calcium chloride dihydrate
Proti-Ace 2 Reagent 3* (Qty 3):
• 1 mg/ml Pepsin, 1 mM Hydrochloric acid
Proti-Ace 2 Reagent 4* (Qty 3):
• 1 mg/ml Thermolysin, 10 mM Calcium acetate hydrate pH 8.0, 50 mM Sodium chloride
Proti-Ace 2 Reagent 5* (Qty 3):
• 1 mg/ml Bromelain, 5 mM HEPES pH 7.5, 2 mM L-Cysteine, 1 mM Ethylenediaminetetraacetic acid disodium salt dihydrate
Proti-Ace 2 Reagent 6* (Qty 3):
• 1 mg/ml Actinase, 5 mM HEPES pH 7.5, 10 mM Sodium chloride

* When 100 µl of deionized water is added to each Proti-Ace 2 freeze dried reagent

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